Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC

Table 1

Optimized gradient program for the separation of four dyes by HPLC-UV, at a flow rate of 1.0 mL min⁻¹.

t (min)	A (%)	B (%)	C (%)
0–12	100	0	0
12–17.6	52.5	9.5	38
17.6–21	0	20	80
21–23	100	0	0