Research Article

Laccase Inhibition by Mercury: Kinetics, Inhibition Mechanism, and Preliminary Application in the Spectrophotometric Quantification of Mercury Ions

Figure 4

Calibration plot for the initial oxidation rate of 200 μM CA per 10 TvL·μg·mL−1 as a function of mercury concentration. The kinetics were obtained in acetate buffer (0.1 M, pH 4.5), monitoring the enzymatic oxidation of CA at 410 nm with 2-minute incubation.