Laccase Inhibition by Mercury: Kinetics, Inhibition Mechanism, and Preliminary Application in the Spectrophotometric Quantification of Mercury Ions

Table 2

Kinetic parameters for the system TvL-CA as a function of the mercury(II) concentration ().

[Hg(II)] (mM)	Km (μM)	Vmax (μM·s^−1)	Kcat (μMmL·μg^−1)	kcat/Km (mL·μg^−1)
0	43 ± 4	90 ± 3	9.0 ± 0.3	0.28 ± 0.03
0.01	46 ± 3	95 ± 3	9.5 ± 0.2	0.20 ± 0.01
0.05	49 ± 3	88 ± 2	8.8 ± 0.3	0.20 ± 0.02
0.1	57 ± 4	79 ± 2	7.9 ± 0.2	0.13 ± 0.01
1	53 ± 4	54 ± 2	5.4 ± 0.2	0.11 ± 0.01
2	55 ± 5	53 ± 2	5.3 ± 0.2	0.10 ± 0.01
3	56 ± 6	52 ± 5	5.2 ± 0.5	0.09 ± 0.01
4	54 ± 5	53 ± 3	5.3 ± 0.3	0.10 ± 0.01