Microfluidic Preparation of Liposomes Using Ethyl Acetate/n-Hexane Solvents as an Alternative to Chloroform

Table 1

Physicochemical properties of chloroform and nonhalogenated solvents.

	Chloroform	n-Hexane	Ethyl acetate	Acetone	Isopropanol	Methanol	Diethyl ether	Methyl acetate	Benzene
Polarity index	4.10	0.10	4.40	5.10	3.90	5.10	2.80	4.40	3.00
Solubility in water (g/100 mL)	0.82	0.00	8.70	Miscible	Miscible	Miscible	6.90	24.40	0.18
Viscosity (cP)	0.57	0.33	0.45	0.32	2.30	0.55	0.22	0.36	0.61
Vapor pressure (kPa)	25.90	17.60	9.73	30.60	6.02	13.02	58.66	23.06	12.70
Lecithin solubility	S	S	S	S	S	I	S	I	S
Allowable concentration (g/kg)	Prohibited	0.01	0.05	0.03	0.05	0.05	Prohibition	Prohibition	Prohibition

S, soluble; I, insoluble.