A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis

Figure 1

Principle of the SA-ELISA method. BaP coating antigens which are covered on the surface of the tubes competed with BaP analytes to combine with the bio-pAb-BaP. The horseradish peroxidase-conjugated streptavidin (SA-HRP) is fixed on the wall through the reaction with bio-pAb-BaP. As the increase of BaP concentration, less bio-pAb-BaP can be attached to the solid carrier. In this case, the amount of SA-HRP is less, and then quantitative analysis could be performed after sufficient colour development.