A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis

Table 1

Cross-reactivity of pAb-BaP with structural analogues.

Analogues	Structure	IC50; (ng mL^−1)	Cross-reactivity; (%)
Benzo(a)pyrene		0.67	100
Benzo[ghi]perylene (BghiP)		30.45	2.2
Indeno[1,2,3-cd]pyrene		23.10	2.9
Perylene		10.31	6.5
Benzo[k]fluoranthene		24.81	2.7
Dibenz[a,h]anthracene		9.71	6.9
Benzo[b]fluoranthene		23.93	2.8
Chrysene		19.14	3.5
Fluoranthene		111.67	0.6
Benz[a]anthracene		18.11	3.7
Pyrene		17.63	3.8
Anthracene		335.12	0.2
Phenanthrene		223.33	0.3
Naphthalene		>3350	<0.02