Research Article
Administration of Vitamin D Metabolites Affects RNA Expression of Xenobiotic Metabolising Enzymes and Function of ABC Transporters in Rats
Table 1
Primers used for quantitative RT-PCR assays.
| | Gene and source | Sequence | Product size |
| GAPDH
NM_017008.4 | for 5′-TTGTCAGCAATGCATCCTGC-3′
rev 5′-CGGCATGTCAGATCCACAAC-3′
Probe: 5′-6FAM-TGGCCCCTCTGGAAAGCTGTGGCG-BBQ | 307 | β-actin
[30] | for 5′-AAGTCCCTCACCCTCCCAAAAG-3′
rev 5′-AAGCAATGCTGTCACCTTCCC-3′ | 97 | BRCP
XM_006236573.3 | for 5′-AGTCCGGAAAACAGCTGAGA-3′
rev 5′-CAAGATGACGTTGTGATGGG-3′ | 235 | CaBP-D9K
NM_012521.2 | for 5′-TGAGCGCTAAGAAATCTCCCG-3′
rev 5′-GCTGGGGAACTCTGACTGAAT-3′ | 125 | CYP3A9
NM_147206.2 | for 5′-CCTGGCTGCTCCTGGTTATC-3′
rev 5′-AAAGGTTTGGGCCCAGGAAT-3′ | 100 | CYP3A18
NM_145782.1 | for 5′-CTACGGTGATGGCATGTGGA-3′
rev 5′-CATTGGCCCAAAACACCGAC-3′ | 178 | CYP3A62
NM_001024232.1 | for 5′-TCTTGCATACCGCCATGGAT-3′
rev 5′-AAAGCTCCGACGGTTTGTGA-3′ | 175 | CYP24A1
NM_201635.2 | for 5′-CTCGGACCCTTGACAAACCA-3′
rev 5′-CCGAATGGGAGATGAGCGAA-3′ | 184 | CYP27B1
NM_053763.1 | for 5′-TACCCCTGCTAAAGGCTGTGA-3′
rev 5′-GAGTTGGGTTCCCGAAACTGG-3′ | 181 | MDR1a
NM_133401.1 | for 5′-TCTGGCGGCCATTATCCAT-3′
rev 5′-TCATACTACGGTTGTTTCCTACATTTG-3′ | 101 | MDR1b
NM_012623.2 | for 5′-TGAATCCCAAAGTGACACTGGT-3′
rev 5′-ATACTTCTGCGAATTGATCTCCTTATT-3′ | 87 | PMCA1b
NM_053311.1 | for 5′-AATGCTTTCCAGAGTGGAGGT-3′
rev 5′-TCGGATACCCCACAGGAGT-3′ | 148 | PXR
NM_052980.2 | for 5′-GCGTCATCAACTTCGCCAAA-3′
rev 5′-TTCTGGAAGCCGCCATTAGG-3′ | 193 | TRPV6
NM_053686.1 | for 5′-GAGCACAGGTTGTGGCTACT-3′
rev 5′-TCCACCCTCAAGAACCAGCG-3′ | 122 | VDR
[24] | for 5′-ACAGTCTGAGGCCCAAGCTA-3′
rev 5′-TCCCTGAAGTCAGCGTAGGT-3′ | 103 |
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