Main Variables Affecting a Chemical-Enzymatic Method to Obtain Protein and Amino Acids from Resistant Microalgae

Table 2

Experimental design and results of the face-centered CCD.

Run	ta	Bc	Te	te	Protein (% of total protein)		FAN (% of total biomass)
					Observed	Predicted	Observed	Predicted
1	−1	−1	−1	−1	61.47	61.36	0.44	0.43
2	+1	−1	−1	−1	71.18	72.14	0.50	0.48
3	−1	−1	−1	+1	66.84	66.13	0.52	0.53
4	+1	−1	−1	+1	79.13	76.91	0.58	0.59
5	0	0	−1	0	57.81	59.17	0.76	0.78
6	−1	+1	−1	−1	35.68	34.85	0.53	0.54
7	+1	+1	−1	−1	40.26	39.13	0.72	0.67
8	−1	+1	−1	+1	43.21	44.65	0.91	0.90
9	+1	+1	−1	+1	47.87	48.93	0.97	1.03
10	0	−1	0	0	73.26	74.07	0.61	0.63
11	−1	0	0	0	55.64	54.21	0.78	0.78
12	+1	0	0	0	61.85	62.66	0.86	0.87
13	0	0	0	−1	56.51	57.63	0.70	0.75
14	0	0	0	+1	65.52	64.92	1.06	0.99
15	0	0	0	0	62.37	61.27	0.87	0.87
16	0	0	0	0	60.39	61.27	0.88	0.87
17	0	0	0	0	60.03	61.27	0.84	0.87
18	0	+1	0	0	48.24	46.83	0.95	0.94
19	−1	−1	+1	−1	65.72	64.64	0.49	0.49
20	+1	−1	+1	−1	77.85	77.25	0.56	0.55
21	−1	−1	+1	+1	67.36	69.41	0.59	0.60
22	+1	−1	+1	+1	81.14	82.03	0.67	0.66
23	0	0	+1	0	65.67	63.37	0.88	0.88
24	−1	+1	+1	−1	36.61	38.13	0.64	0.67
25	+1	+1	+1	−1	43.52	44.24	0.81	0.80
26	−1	+1	+1	+1	48.79	47.93	1.05	1.04
27	+1	+1	+1	+1	54.55	54.05	1.15	1.16