GSH Synthetic Analogue O-Methyl-L-Tyrosinylglutathione Regulates Nrf2-Mediated Expression of GCLc and GCLm

Figure 3

Western blot analysis of Nrf2 concentration in the nuclear and cytoplasmic fractions of HepG2 cells. Cells were treated with 0.1 mM UPF1 or DPBS (control) for indicated time periods. As a positive control of Nrf2, activation cells were incubated with 0.1 mM H₂O₂ for 60 min. Then, the nuclear and cytoplasmic fractions were separated and analysed for Nrf2 concentration using 10% SDS-polyacrylamide gel. Representative western blot bands are inserted under the x-axis at their respective time points with β-Actin as a loading control. Images were analysed using ImageJ software. .