Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Figure 1

Chromatographic separation of four tocopherol homologues on the Kinetex™ C18 column by RP-LC and SFC. The concentration of tocopherol homologues injected in both systems was equal. The chromatographic conditions of the RP-LC method: mobile phase: MeOH; isocratic flow rate: 1 mL/min; column oven temperature: 25°C; room temperature: 22°C; injection volume: 1 μL. The chromatographic conditions of the SFC method—mobile phase: CO₂ : MeOH (99.8 : 0.2, v/v); isocratic flow rate: 4 mL/min; column oven temperature: 25°C; room temperature: 22°C; injection volume: 1 μL; back pressure regulator: 15 MPa.