Research Article

Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Figure 2

Impact of the methanol concentration in the mobile phase on the tocopherol homologues selectivity and sensitivity of the RP-LC-DAD method. The concentration of tocopherol homologues in each injection was equal. The chromatographic conditions: mobile phase: MeOH : H2O (100 : 0–95 : 5, v/v); isocratic flow rate: 1 mL/min; column oven temperature: 40°C; room temperature: 22°C; injection volume: 1 μL.