Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

<div>Impact of the column oven temperature (25 vs 50°C) on the tocopherol homologues selectivity and sensitivity of the SFC-DAD method. The concentration of tocopherol homologues injected in each case was equal. The chromatographic conditions of the SFC method: mobile phase: CO<sub>2</sub> : MeOH (99.8 : 0.2, v/v); isocratic flow rate: 4 mL/min; room temperature: 22°C; injection volume: 1 <i>μ</i>L; back pressure regulator: 15 MPa.</div>

Journal of Chemistry

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Figure 4

Figure 4: Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column 

Figure 4 | Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column 