Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

<div>Impact of the injection volume of standards diluted in methanol on the tocopherol homologues peak shape via SFC-DAD. The concentration of tocopherol homologues in the sample was equal with the exception of grey chromatograms where the sample was diluted 10 times in methanol. The chromatographic conditions of the SFC method: mobile phase: CO<sub>2</sub> : MeOH (99.8 : 0.2, v/v); isocratic flow rate: 4.5 mL/min; column oven temperature: 40°C; room temperature: 22°C; back pressure regulator: 15 MPa.</div>

Journal of Chemistry

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Figure 5

Figure 5: Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column 

Figure 5 | Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column 