Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Figure 6

Chromatograms of the tocopherol homologues separation in four selected fruit seed samples via SFC-DAD. The 0.1 g of each fruit seed sample was used for tocopherols extraction and finally diluted in 0.5 mL of methanol. The chromatographic conditions of the SFC method: mobile phase: CO₂ : MeOH (99.8 : 0.2, v/v); isocratic flow rate: 4.5 mL/min; column oven temperature: 40°C; room temperature: 22°C; injection volume: 2 μL; back pressure regulator: 15 MPa.