Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Table 1

Separation parameters of the four tocopherol homologues on the Kinetex C18 column with the optimized SFC chromatographic conditions^∗.

Compounds	Retention time, TR (min)	Retention factor, k	Selectivity factor, αB/A	Resolution, Rs	Number of theoretical plates, N
α-T	1.152	3.28	—	—	5523.8
β-T	1.354	4.03	1.23	3.015	5658.4
γ-T	1.459	4.42	1.10	1.400	5598.1
δ-T	1.646	5.12	1.16	2.267	5727.2

Details of the SFC separation conditions: flow rate: 4.5 mL/min; column dead time (T₀): 0.269 min; mobile phase: CO₂ : MeOH (99.8 : 0.2, v/v); column oven temperature: 40°C; room temperature: 22°C; back pressure regulator: 15 MPa.