Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Table 2

Linearity, limit of detection (LOD), and limit of quantification (LOQ) of the developed method employing the Kinetex C18 column and the SFC-DAD system^a.

	Tocopherols
	α-T	β-T	γ-T	δ-T
Standard solutions (R^2)	0.9985 ± 0.0008	0.9988 ± 0.0005	0.9990 ± 0.0006	0.9991 ± 0.0007
Slope and y intercept	y = 41.458x − 1349.4	y = 70.363x − 2807.5	y = 72.367x − 3144.6	y = 70.056x − 2939.1
LOD (ng/μL)	29	32	30	27
LOQ (ng/μL)	88	97	90	83

x, concentration (ng); y, peak area (mAU). ^aTocopherols presented as the absolute amount injected and dissolved in 1 μL of solvent.