Research Article
Aerobic Biodegradation of Four Groups of Steroid Hormones in Activated Sludge
Table 1
Optimized instrumental and MRM conditions of the target compounds.
| Analyte | Abbreviation | Mode | Precursor ion | MRM transition | CV (V) | CE (eV) | IDL (μg/L) |
| Estrogens | | | | | | | | Estrone | E1 | ESI+ | [M-OH]+ | 271 > 147a | 18 | 10 | 2.0 | 271 > 197b | 17 | α-Estradiol | αE2 | ESI+ | [M-OH]+ | 255 > 159 | 35 | 20 | 0.20 | 255 > 133 | 20 | β-Estradiol | βE2 | ESI+ | [M-OH]+ | 255 > 159 | 35 | 20 | 1.0 | 255 > 133 | 20 | Ethinylestradiol | EE2 | ESI+ | [M-OH]+ | 279 > 133 | 24 | 16 | 1.0 | 279 > 159 | 21 | Androgens | | | | | | | | Testosterone | T | ESI+ | [M + H]+ | 289 > 97 | 35 | 20 | 2.0 | 289 > 109 | 20 | Methyl-testosterone | MT | ESI+ | [M + H]+ | 303 > 97 | 22 | 22 | 1.8 | 303 > 109 | 15 | epitestosterone | ET | ESI+ | [M + H]+ | 289 > 97 | 35 | 20 | 1.5 | 289 > 109 | 20 | Trenbolone | TBL | ESI+ | [M + H]+ | 271 > 253 | 22 | 18 | 1.5 | 271 > 199 | 22 | Boldenone | BOL | ESI+ | [M + H]+ | 287 > 121 | 24 | 22 | 3.3 | 287 > 135 | 15 | Dehydroepiandrosterone | DEHA | ESI+ | [M + H]+ | 271 > 253 | 28 | 24 | 2.4 | 271 > 199 | | cis-Androsterone | cis-ADR | ESI+ | [M + H]+ | 273 > 255 | 20 | 15 | 5.0 | 273 > 273 | 10 | Progestogens | | | | | | | | Progesterone | P | ESI+ | [M + H]+ | 351 > 109 | 35 | 20 | 1.3 | 351 > 97 | 16 | 21α-Hydroxyprogesterone | 21α-HPT | ESI+ | [M + H]+ | 331 > 108 | 28 | 22 | 1.0 | 331 > 97 | 21 | 17α-Hydroxyprogesterone | 17α-HPT | ESI+ | [M + H]+ | 331 > 108 | 28 | 22 | 1.0 | 331 > 97 | 21 | 19-Nortestosterone | 19-NT | ESI+ | [M + H]+ | 275 > 257 | 28 | 15 | 0.15 | 275 > 239 | 15 | Medroxyprogesterone acetate | MPA | ESI+ | [M + H]+ | 387 > 327 | 28 | 16 | 0.20 | 387 > 285 | 16 | 19-Norethindrone | 19-NTD | ESI+ | [M + H]+ | 299 > 231 | 24 | 17 | 3.0 | 299 > 109 | 24 | Melengestrol acetate | MGA | ESI+ | [M + H]+ | 397 > 337 | 26 | 15 | 0.05 | 397 > 279 | 20 | Glucocorticoids | | | | | | | | Cortisol | CRL | ESI- | [M-CH2COO]− | 421 > 331 | 30 | 16 | 0.40 | 421 > 361 | 16 | Cortisol 17 acetate | 17-CRLA | ESI- | [M-CH2COO]− | 463 > 403 | 27 | 11 | 0.17 | 463 > 373 | 16 | Cortisol 21 acetate | 21-CRLA | ESI- | [M-CH2COO]− | 463 > 403 | 27 | 11 | 0.26 | 463 > 373 | 16 | Cortisol 17-valerate | 17-CRLV | ESI- | [M-CH2COO]− | 505 > 455 | 20 | 10 | 0.12 | 505 > 331 | 16 | Cortisol 21-valerate | 21-CRLV | ESI- | [M-CH2COO]− | 505 > 455 | 20 | 10 | 0.18 | 505 > 331 | 16 | Betamethasone | BET | ESI- | [M-CH2COO]− | 451 > 361 | 27 | 16 | 0.44 | 451 > 391 | 11 | Dexamethasone | DEX | ESI- | [M-CH2COO]− | 451 > 361 | 27 | 16 | 0.85 | 451 > 391 | 11 | Betamethasone acetate | BETA | ESI- | [M-CH2COO]− | 493 > 433 | 20 | 12 | 0.33 | 493 > 353 | 18 | Dexamethasone acetate | DEXA | ESI- | [M-CH2COO]− | 493 > 433 | 20 | 12 | 0.48 | 493 > 353 | 18 | Cortisone | COR | ESI- | [M-CH2COO]− | 419 > 331 | 30 | 16 | 0.60 | 419 > 361 | 16 | Cortisone acetate | CORA | ESI- | [M-CH2COO]− | 461 > 371 | 27 | 25 | 0.14 | 461 > 401 | 16 |
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For all the compounds, a is the first MRM transition used for quantification, and b is the second MRM transition used for verification.
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