Journal of Chemistry

Research Article

Aerobic Biodegradation of Four Groups of Steroid Hormones in Activated Sludge

Table 1

Optimized instrumental and MRM conditions of the target compounds.


Analyte	Abbreviation	Mode	Precursor ion	MRM transition	CV (V)	CE (eV)	IDL (μg/L)

Estrogens
Estrone	E1	ESI+	[M-OH]⁺	271 > 147^a	18	10	2.0
				271 > 197^b		17
α-Estradiol	αE2	ESI+	[M-OH]⁺	255 > 159	35	20	0.20
				255 > 133		20
β-Estradiol	βE2	ESI+	[M-OH]⁺	255 > 159	35	20	1.0
				255 > 133		20
Ethinylestradiol	EE2	ESI+	[M-OH]⁺	279 > 133	24	16	1.0
				279 > 159		21
Androgens
Testosterone	T	ESI+	[M + H]+	289 > 97	35	20	2.0
				289 > 109		20
Methyl-testosterone	MT	ESI+	[M + H]+	303 > 97	22	22	1.8
				303 > 109		15
epitestosterone	ET	ESI+	[M + H]+	289 > 97	35	20	1.5
				289 > 109		20
Trenbolone	TBL	ESI+	[M + H]+	271 > 253	22	18	1.5
				271 > 199		22
Boldenone	BOL	ESI+	[M + H]+	287 > 121	24	22	3.3
				287 > 135		15
Dehydroepiandrosterone	DEHA	ESI+	[M + H]+	271 > 253	28	24	2.4
				271 > 199
cis-Androsterone	cis-ADR	ESI+	[M + H]+	273 > 255	20	15	5.0
				273 > 273		10
Progestogens
Progesterone	P	ESI+	[M + H]+	351 > 109	35	20	1.3
				351 > 97		16
21α-Hydroxyprogesterone	21α-HPT	ESI+	[M + H]⁺	331 > 108	28	22	1.0
				331 > 97		21
17α-Hydroxyprogesterone	17α-HPT	ESI+	[M + H]⁺	331 > 108	28	22	1.0
				331 > 97		21
19-Nortestosterone	19-NT	ESI+	[M + H]⁺	275 > 257	28	15	0.15
				275 > 239		15
Medroxyprogesterone acetate	MPA	ESI+	[M + H]⁺	387 > 327	28	16	0.20
				387 > 285		16
19-Norethindrone	19-NTD	ESI+	[M + H]⁺	299 > 231	24	17	3.0
				299 > 109		24
Melengestrol acetate	MGA	ESI+	[M + H]⁺	397 > 337	26	15	0.05
				397 > 279		20
Glucocorticoids
Cortisol	CRL	ESI-	[M-CH₂COO]⁻	421 > 331	30	16	0.40
				421 > 361		16
Cortisol 17 acetate	17-CRLA	ESI-	[M-CH₂COO]⁻	463 > 403	27	11	0.17
				463 > 373		16
Cortisol 21 acetate	21-CRLA	ESI-	[M-CH₂COO]⁻	463 > 403	27	11	0.26
				463 > 373		16
Cortisol 17-valerate	17-CRLV	ESI-	[M-CH₂COO]⁻	505 > 455	20	10	0.12
				505 > 331		16
Cortisol 21-valerate	21-CRLV	ESI-	[M-CH₂COO]⁻	505 > 455	20	10	0.18
				505 > 331		16
Betamethasone	BET	ESI-	[M-CH₂COO]⁻	451 > 361	27	16	0.44
				451 > 391		11
Dexamethasone	DEX	ESI-	[M-CH₂COO]⁻	451 > 361	27	16	0.85
				451 > 391		11
Betamethasone acetate	BETA	ESI-	[M-CH₂COO]⁻	493 > 433	20	12	0.33
				493 > 353		18
Dexamethasone acetate	DEXA	ESI-	[M-CH₂COO]⁻	493 > 433	20	12	0.48
				493 > 353		18
Cortisone	COR	ESI-	[M-CH₂COO]⁻	419 > 331	30	16	0.60
				419 > 361		16
Cortisone acetate	CORA	ESI-	[M-CH₂COO]⁻	461 > 371	27	25	0.14
				461 > 401		16

For all the compounds, ^a is the first MRM transition used for quantification, and ^b is the second MRM transition used for verification.