A Synthetic Pan-Aurora Kinase Inhibitor, 5-Methoxy-2-(2-methoxynaphthalen-1-yl)-4H-chromen-4-one, Triggers Reactive Oxygen Species-Mediated Apoptosis in HCT116 Colon Cancer Cells

Figure 2

Effect of DK1913 on the induction of cell cycle arrest and apoptosis. (a) HCT116 cells (1 × 10³ cells/sample) were treated with different concentrations of DK1913 for 24 h. Cell viability was measured using a cell counting kit-8. Data are presented as means ± SD (n = 3). NS, not significant (); ; ; by Dunnett’s multiple comparisons test. (b) HCT116 cells (3 × 10⁵ cells/sample) were treated with either vehicle (dimethyl sulfoxide, DMSO) or 5 μM DK1913 for 24 h. After staining with propidium iodide (PI), cellular DNA contents were measured with a NucleoCounter NC-3000 cytometer. M1, sub-G1 phase; M2, G1 phase; M3, S phase; M4, G2/M phase. 2N: diploid DNA content; 4N: tetraploid DNA content. (c) HCT116 cells were treated with 5 μM DK1913 for 48 h and then stained with propidium iodide (PI) and FITC-Annexin V. Fluorescence intensities were analyzed using a NucleoCounter NC-3000 cytometer. Scatter plots (top panels) show FITC-Annexin V intensity (x-axis) versus PI intensity (y-axis). Histograms (bottom panels) show FITC-Annexin V intensity (x-axis) versus the percentage of the cell population (y-axis). M1, Annexin V negative; M2, Annexin V positive.

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