A Synthetic Pan-Aurora Kinase Inhibitor, 5-Methoxy-2-(2-methoxynaphthalen-1-yl)-4H-chromen-4-one, Triggers Reactive Oxygen Species-Mediated Apoptosis in HCT116 Colon Cancer Cells

Figure 3

Effect of DK1913 on the generation of reactive oxygen species (ROS) in HCT116 cells. (a) HCT116 cells were incubated with 10 μM DCF-DA for 60 min, followed by incubation with 5 μM DK1913 for different periods (0, 1, 6, and 12 h). DCF fluorescence was assessed using flow cytometry performed on a FACSCalibur flow cytometer. (b) HCT116 cells were incubated with 10 μM DCF-DA for 60 min, followed by incubation with 5 μM DK1913 for 12 h. Intracellular ROS were visualized using an EVOSf1 fluorescence microscope (scale bar, 400 μm).

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