Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Plasmids</td><td class="align_center">Relevant characteristics</td><td class="align_center">Sources</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">pGUC</td><td class="align_center">Ampr E. coli-G. oxydans shuttle vector</td><td class="align_center">This study</td></tr><tr><td class="align_left">pGUC-tufB-sdh-GGGGS-sndh</td><td class="align_center">pGUC containing tufB-sdh-GGGGS-sndh</td><td class="align_center">This study</td></tr><tr><td class="align_left">pGUC-tufB-cutA</td><td class="align_center">pGUC containing tufB-cutA</td><td class="align_center">This study</td></tr><tr><td class="align_left">pGUC-tufB-SH3-sdh-GGGGS-sndh-tufB-SH3lig-(GGGGS)2-cutA</td><td class="align_center">pGUC containing tufB-SH3-sdh-GGGGS-sndh-tufB-SH3lig-(GGGGS)2-cutA</td><td class="align_center">This study</td></tr><tr><td class="align_left">pGUC-tufB-SH3-sdh-GGGGS-sndh-tufB-SH3lig-(GGGGS)2-cutA-tufB-pqqABCDE</td><td class="align_center">pGUC containing tufB-SH3-sdh-GGGGS-sndh-tufB-SH3lig-(GGGGS)2-cutA-tufB-pqqABCDE</td><td class="align_center">This study</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Journal of Chemistry

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Table 1

Table 1: Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid

Table 1 | Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid 