Determination of Pesticide Residue in Brinjal Sample Using HPTLC and Developing a Cost-Effective Method Alternative to HPLC

Table 1

R_f and MDQ values of four TLC validation methods.

Pesticide	Rf	RRf	MDQ (ng)	Coefficient of variation
o-tolidine + potassium iodide (o-TKI) method
Oxamyl	0.2 ± 0.001	0.32	90	0.52
Diuron	0.37 ± 0.018	0.59	30	4.89
Atrazine	0.62 ± 0.018	1	20	2.90
Dimethoate	0.27 ± 0.002	0.43	100	0.73
Imidacloprid	0.23 ± 0.002	0.37	130	0.88
Fungi spore method, FAN
Captan	0.63 ± 0.002	1	20	0.32
Fenarimol	0.51 ± 0.004	0.80	1	0.20
Procloraz	0.37 ± 0.001	0.60	20	0.27
Imazolil	0.15 ± 0.001	0.23	5	0.68
Thiophanate-e-methyl	0.58 ± 0.002	0.93	50	0.33
Horse blood serum method, Eacl
Oxamyl	0.18 ± 0.002	0.28	1.2	1.10
Parathion-methyl	0.58 ± 0.010	0.90	9	1.72
Methomyl	0.05 ± 0.006	0.07	75	12.00
Methidathion	0.63 ± 0.001	0.98	100	0.16
Phosphamidon	0.23 ± 0.001	0.35	50	0.44
Malathion	0.64 ± 0.002	1	100	0.31
Dichlorvos	0.50 ± 0.002	0.78	2.0	0.40
Photosynthesis inhibition method, Hill reaction
Atrazine	0.66 ± 0.01	1	2	1.51
Linuron	0.62 ± 0.007	0.93	2	1.12
Chlortoluron	0.44 ± 0.006	0.66	3.5	1.36
Isoproturon	0.41 ± 0.008	0.62	3	1.95
Thiabendazole	0.40 ± 0.02	0.60	25	5.00

Values are mean of three replicates. SD:  standard deviation. MDQ: maximum detected quantity. C.V: coefficient of variation.