Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Table 2

Diclofenac and its biotransformation product defined by their retention times, measured and theoretical protonated molecules (fragment ions), and formula.

Compound	tret (min)	[M + H]^+ (m/z measured)	[M + H]^+ (m/z theoretical)	Mass error (mDa)	Formula	Mass difference
Diclofenac	5.0	296.0243	296.0240	0.3	C14H11Cl2NO2	0.00000
4′-Hydroxy-diclofenac	4.0	312.0209	312.0189	2.0	C14H11Cl2NO3	15.9966