Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Table 4

EC₅₀ values obtained during the evaluation of the ecotoxicity of the medium used for degradation experiments.

Compounds (initial concentration)	Test protocol	EC50 after 30 min of exposure Mean [% min–% max]
Tryptone/peptone from casein (6.0 g·L^−1); Casein hydrolysate standard (3.0 g·L^−1); Yeast extract (3.0 g·L^−1); Glucose (1.0 g·L^−1)	Basic test 45% (n = 3)	31.9% [20.3–52.9]
Glucose (1.0 g·L^−1)	Screening test 81.9% (n = 2)	8.14% [4.81–11.5]
Tryptone/peptone from casein (6.0 g·L^−1); Casein hydrolysate standard (3. 0 g·L^−1); Yeast extract (3.0 g·L^−1)	Screening test 81.9% (n = 2)	20.7% [4.36–37.0]
Tryptone/peptone from casein (6.0 g·L^−1); Casein hydrolysate standard (3. 0 g·L^−1); Yeast extract (3.0 g·L^−1); NaHCO3 (1.0 g·L^−1) + glycerol (3.0 g·L^−1)	Screening test 81.9% (n = 2)	17.0% [0.39–33.7]
Tryptone/peptone from casein (6.0 g·L^−1)	Screening test 81.9% (n = 2)	40.3% [34.5–46.1]
Casein hydrolysate standard (3. 0 g·L^−1)	Screening test 81.9% (n = 2)	26.6% [19.8–33.3]
Yeast extract (3.0 g·L^−1)	Screening test 81.9% (n = 2)	18.8% [33.1–45.5]