Green Synthesis of Gold and Iron Nanoparticles for Targeted Delivery: An In Vitro and In Vivo Study

Figure 3

(a) Minimum inhibitory concentration of gold and iron nanoparticles against bacterial strains; S.A Staphylococcus aureus, M.L Micrococcus luteus, B. sub Bacillus subtilis, B. sep Bacillus septica, E. coli Escherichia coli, E.A Enterobacter aerogenes, S.T Staphylococcus aureus. (b) IC50 values of antioxidant potential of gold iron nanoparticles (FeNPs and AuNPs) assessed by DPPH free radical scavenging assay. (c) Brine shrimp cytotoxic assay, percent viability of brine shrimps at 50 μg/mL dose of gold and iron nanoparticles (FeNPs and AuNPs). (d) (A) DNA damage assay of gold nanoparticles (AuNPs), (B) DNA damage assay of iron nanoparticles (FeNPs); L: DNA ladder (1 Kb), P: pBR322 plasmid, X: pBR322 plasmid treated with FeSO₄ and H₂O₂ (positive control), 1: pBR322 plasmid + 1000 μg/mL of nanoparticles, control for the prooxidant effect, 2: plasmid + 1000 μg/mL of nanoparticles + FeSO₄ + H₂O₂, 3: plasmid + 100 μg/mL of nanoparticles + FeSO₄ + H₂O₂, 4: plasmid + 10 μg/mL of nanoparticles + FeSO₄ + H₂O₂. Data represent the mean of triplicate with statistical significance a: , b: .

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