A Rapid and Sensitive UHPLC-MS/MS Method for Determination of 2, 3, 8-Trimethylellagic, a Potent Active Compound from Sanguisorba officinalis L., and Its Application in the Pharmacokinetic Study within Thrombocytopenia Rats

Table 5

Pharmacokinetic parameter of TMEA in rat after intragastric administration of four groups (n = 6).

Parameter	NG	TG	TMG	TOG
AUC0 − t(ng·h/mL)	752.68 ± 144.33	1185.67 ± 178.36	886.47 ± 190.72^▲	1895.37 ± 286.73^▲▲
AUC0 − ∞(ng·h/mL)	833.87 ± 169.63	1462.82 ± 346.33	1121.94 ± 188.25	3023.54 ± 983.08^▲▲
MRT (h)	28.85 ± 12.25	40.15 ± 10.22	45.53 ± 23.66	78.70 ± 58.83
t1/2 (h)	23.61 ± 16.09	34.80 ± 14.36	36.50 ± 19.41	61.43 ± 45.40
Tmax (h)	3.16 ± 0.40	2.16 ± 0.40	3.00 ± 0.00^▲▲	2.00 ± 0.00
Vz/F (mL/kg)	160604.50 ± 89246.64	133606.50 ± 23554.64	186729.66 ± 84733.41	109132.16 ± 49342.68
CL/F (mL/h/kg)	4950.33 ± 913.25	2845.83 ± 570.44	3652.00 ± 628.26^▲	1412.33 ± 337.40^▲▲
Cmax (ng/mL)	68.03 ± 10.75	115.92 ± 14.53	89.92 ± 5.34^▲▲	198.36 ± 51.53^▲▲

Abbreviations: NG, normal group; TG, TMEA group; TMG, TMEA-DMAG group; TOG, TMEA-DOMA group; AUC_0 − t, area under the concentration–time curve from 0 to 72 h; AUC_{0 − ∞}, area under the concentration–time curve from 0 to time infinite; MRT, mean residence time; t_1/2, half-life of elimination; T_max, time to achieve maximum concentration; V_z/F, volume of distribution; CL/F, clearance; C_max, maximum value of concentration; , significant compared with NG, , compared with TG.