Optimization of Spectrophotometric and Fluorometric Assays Using Alternative Substrates for the High-Throughput Screening of Lipase Activity

Figure 1

Effects of substrate (p-nitrophenyl palmitate, p-NPP) concentration on the catalytic activity of lipases. The substrate concentration was in the range of 1–5 mM. Error bars indicate the standard error of the mean. Superscripts (a–e) above the bars denote significant differences in catalytic activity ( , Duncan’s multiple range test on ANOVA). CVL, Chromobacterium viscosum lipase (0.01 mg/mL); PFL, Pseudomonas fluorescens lipase (0.001 mg/mL); PPL, porcine (Sus scrofa) pancreatic lipase (10 mg/mL); WGL, wheat germ (Triticum aestivum) lipase (1 mg/mL).