Optimization of Spectrophotometric and Fluorometric Assays Using Alternative Substrates for the High-Throughput Screening of Lipase Activity

Figure 6

Effects of operating temperature on the catalytic activity of lipases against 4-methylumbelliferyl oleate as a substrate. The operating temperature was in the range of 25–55°C. Error bars indicate the standard error of the mean. Superscripts (a–f) above the bars denote significant differences in catalytic activity (, Duncan’s multiple range test on ANOVA). CVL, Chromobacterium viscosum lipase (0.1 μg/mL); PFL, Pseudomonas fluorescens lipase (0.1 μg/mL); PPL, porcine (Sus scrofa) pancreatic lipase (10 mg/mL); WGL, wheat germ (Triticum aestivum) lipase (1 mg/mL).