Research Article
Optimization of Spectrophotometric and Fluorometric Assays Using Alternative Substrates for the High-Throughput Screening of Lipase Activity
Table 1
Lipase activity in crude protein extracts from domestic agricultural products (spectrophotometric assay).
| | Classification | Items | Concentration (mg/mL) | Activity (μU/mL) | Specific activity (μU/mg protein) | | Name | Specimen |
| | Mushrooms | Shiitake mushroom (Lentinula edodes) | Pileus | 6.0080 | 148.0607 | 24.6439 | | Stalk | 4.6380 | 73.5127 | 15.8501 | | White mushroom (Agaricus bisporus) | Whole | 7.4760 | 801.3916 | 107.1952 | | Lion’s mane mushroom (Hericium erinaceus) | Whole | 4.5490 | 218.4672 | 48.0253 | | King oyster mushroom (Pleurotus eryngii) | Pileus | 5.1730 | 633.4164 | 379.4340 | | Stalk | 4.2960 | 379.4340 | 633.4164 |
| | Vegetables | Soybean sprout (Glycine max) | Cotyledon | 16.4810 | N.D | N.D | | Hypocotyl | 5.0780 | 272.3075 | 53.6249 | | Onion (Allium cepa) | Whole | 4.4080 | 197.7594 | 44.8638 | | Pumpkin (Cucurbita moschata) | Whole | 5.2280 | 955.6646 | 182.7974 | | Chili pepper (Capsicum annuum) | Whole | 11.0353 | N.D. | N.D. | | Broccoli (Brassica oleracea) | Crown | 5.0830 | 1653.8623 | 325.3713 | | Stalk | 7.0280 | 912.8685 | 129.8902 | | Leaf | 13.0710 | 138.3971 | 10.5881 |
| | Cereals | Job’s tears (Coix lacryma-jobi) | Whole | 8.2480 | 634.6938 | 76.9512 | | Buckwheat (Fagopyrum esculentum) | Whole | 9.2220 | 980.1688 | 106.2859 | | Pigweed (Amaranthus sp.) | Whole | 4.4660 | 294.3958 | 65.9193 | | Sorghum (Sorghum bicolor) | Whole | 4.0380 | 190.5117 | 47.1797 | | Brown rice (Oryza sativa) | Whole | 5.2450 | 666.7909 | 127.1289 |
| | Nuts/seeds | Chili pepper seed (Capsicum annuum) | Whole | 5.6420 | 1941.3555 | 344.0900 | | Sunflower seed (Helianthus annuus) | Whole | 5.8240 | 768.2591 | 131.9126 | | Peanut (Arachis hypogaea) | Whole | 5.4650 | 630.8974 | 115.4433 |
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N.D, not detected.
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