Osteogenic Activity of Lupeol Isolated from Clinacanthus nutans Lindau: Activity and Mode of Action

Figure 2

Cell viability of MC3T3-E1 cells in the presence of lupeol. MC3T3-E1 cells were seeded in the MEMα medium and then treated with lupeol at different concentrations. After incubation and treatment in 96 h (4 days), the medium was removed, and the cells were incubated with 5 µg/mL MTT solution for 4 h at 37°C in 5% CO₂. Data of relative cell viability are calculated as percentage compared to the nontreated group (control). Results are presented as mean ± SD, n = 3.