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S. no. | Microorganisms and enzymes | Substrate | Optimum temperature (°C) | Optimum pH | Mol. wt (kDa) | Application | Reference |
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1 | Pseudomonas putida F6 (cell-free extracts laccase) | Syringaldazine (SGZ) | 30 | 7.0 | 59 | Degradation of synthetic dyes | [7] |
2 | Streptomyces cyaneus (cell-free extracts laccase) | 2,2′-Azino-bis-(3-ethylbenzothiazoline -6-sulfonic acid (ABTS) | 60 | 4.5 | — | Oxidation of micropollutants such as BPA, DFC, and MFA | [8] |
3 | Geobacillus thermocatenulatus (cell-free extracts laccase) | ABTS | 60 | 4.5 | 42.5 65 | Decolorization of textile dyes, especially Congo red and bromophenol blue | [9] |
4 | Bacillus safensis (cell-free extracts laccase) | — | 37 | 6.2 | — | Decolorization of commercially available dyes | [10] |
5 | A. gonensis (cell-free extracts laccase) | ABTS | 60 | 5.0 | 160 | Bioremediation of wastewater | [11] |
6 | Rhodococcus rhodochrous (cell-free extracts P450) | Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) | 30 | 7.2 | — | Degradation of RDX | [12] |
7 | Bacillus megaterium (purified P450) | 1-Monochloro-dibenzo-p-dioxin (1-MCDD), 2,3-dichlorodibenzo-p-dioxin (2,3-DCCD), 2,3,7-trichloro-dibenzo-p-dioxin (2,3,7-triCDD) | 30 | 7.5 | 120 | Hydroxylation of PCCDs | [13] |
8 | Mycobacterium sp. (cell-free extracts P450) | — | 30 | 6.6 | — | Biodegradation of morpholine | [14] |
9 | Bacillus sp; Geobacillus (purified amylase) | Diethylethanolamine (DEAE) | 90 | 8.0 | — | Starch liquefaction | [15] |
10 | Bacillus subtilis (cell-free extracts lipase) | Olive oil | 45 | 7.0 | — | Bioremediation of wastewater | [16] |
11 | Bacillus pumilus (cell-free extracts lipase) | Palm oil | 50 | 7.0 | — | Degradation of palm oil containing industrial wastewater | [17] |
12 | Chromobacterium viscosum (cell-free extracts lipase) | — | 37 | 7.0 | — | Degradation of PBSA, PCL, PBS | [18] |
13 | Bacillus subtilis (cell-free extracts lipase) | Olive oil | 37 | 8.0 | — | Removal of trough oil or grease stains from detergent | [19] |
14 | Sphingobacterium sp. strain S2 (cell-free extracts lipase) | Chromogenic PNPL | 37 | 7.0 | 40 | Degradation of PLA | [20] |
15 | Pseudomonas putida (cell-free extracts dehydrogenase) | 4-Hydroxybenzaldehyde and 4-hydroxy-3-methylbenzaldehyde | 30 | 8 | — | Catabolism of 2,4-xylenol | [21] |
16 | S. rhizophila (cell-free extracts dehydrogenase) | Polyvinyl alcohol and vinyl alcohol oligomer | 30 | 7.2 | — | Polyvinyl alcohol degradation | [22] |
17 | Bacillus subtilis (cell-free extracts serine protease) | Feather culture medium | 25 | 5–8 | 25.4 | Degradation of casein and feather | [23] |
18 | Chryseobacterium sp. strain kr6 (cell-free extracts protease) | Chicken feathers | 30 | 8 | — | Complete degradation of feathers | [24] |
19 | Bacillus pumilus keratinase (cell-free extracts protease) | Chicken feathers | 37 | 8 | — | Complete degradation of feathers | [25] |
20 | Streptomyces thermoviolaceus (cell-free extracts protease) | Muscle, collagen, hair, nail, feathers | 55 | 8 | 40 | Hydrolyze fibrin, muscle, collagen, nail, and hair | [26] |
21 | Thermoanaerobacter keratinophilus (purified extracellular protease) | Anaerobic complex medium with chicken feathers, merino wool, or human hair | 85 | 8 | 135 | Degradation of keratin fibers | [27] |
22 | B. subtilis (purified protease) | Casein | 50 | 8 | 44 | Deproteinization of crustacean wastes | [28] |
23 | Ancylobacter aquaticus (purified dehalogenase) | Monochloroacetate | 37 | 9 | 25 | Degradation of halogen acid ester | [29] |
24 | Bacillus sp. (cell-free extracts dehalogenase) | 2,4,6-Trinitrobromophenol (TBP) | 37 | 7.5 | — | Degradation of TBP | [30] |
25 | Ochrobactrum (purified dehalogenase) | TBBPA | 35 | | 117 | Degradation of TBBPA | [31] |
26 | Pseudomonas sp. TL (purified dehalogenase) | 2-Chloropropionoate | 65 | 9.5 | 28 | Degradation of halogen acid | [32] |
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