Microbial Enzymes Used in Bioremediation

Table 3

Microbial enzyme produced by recombinant DNA technology for enhanced bioremediation.

S.no.	Recombinant enzyme	Bacteria host	Origin	Expression vector	Gene size/enzyme	Application	Reference
1	Laccase CotA	E. coli DH5	B. subtilis	pMD18-T	1542 bp	Degradation of synthetic dyes	[53]
2	Laccase CueO	P. pastoris GS115	E. coli K12	pHBM905BDM	1470 bp/55 kDa	Decolorization of wastewater from the textile printing factory	[54]
3	Laccase (Fmb-rL103)	E. coli BL21 (DE3)	B. vallismortis fmb-103	pMD19-T-lac103	1542 bp/70 kDa	Degradation of MG (a triphenylmethane dye)	[55]
4	CYP105D1	Acinetobacter calcoaceticus	Streptomyces griseus	pSP19g10L	1500 bp	Degradation of pollutant herbicides	[40]
5	CYP153A6	Pseudomonas putida GPo12	Mycobacterium sp. HXN-1500	pGEc47B	—	Hydroxylation of alkanes to 1-alkanols	[56]
6	CYP153	E. coli	Acinetobacter sp. EB104	pUC18	1494 bp/56 kDa	Hydroxylation of unsubstituted alkanes	[57]
7	CYP154H1	E. coli C43 (DE3)	Thermobifida fusca	pIT2-MCS	NR/48kDa	Hydroxylation of small aliphatic and aromatic compounds to alcohol and epoxide	[58]
8.	CYP151A2	E. coli SG13009	Mycobacterium sp. strain RP1	pQE30	3900 bp/45 kDa	Degradation of secondary amines by ring cleavage	[59]
9	Dehydrogenase	E. coli	Pseudomonas sp. Strain WBC-3	pET29b	38 kDa	Catabolism of para-nitro phenol (PNP) to Krebs cycle intermediates	[60]
10	Aldehyde dehydrogenase	E. coli ER2566	Bacillus cereus	pRSFDuet-1	54 kDa	Regulate harmful metabolic intermediate aldehydes	[61]
11	Dehydrogenase	E. coli	Pseudomonas putida S12	pET28	30 kDa	Catalyzes the NAD^+-dependent oxidation of phenylacetaldehyde to phenylacetic acid in the styrene catabolic and detoxification pathway	[62]
12	Dehydrogenase	E. coli	Azoarcus evansii KB740	pMal-c2x	54 kDa	Oxidizes ring cleavage product 3,4-dehydroadipyl-CoA semialdehyde	[63]
13	Dehydrogenase	E. coli	Rhodococcus sp. P14	pET-32a	45 kDa	Bioremediation of steroids	[64]
14	Dehalogenase	E. coli BL21 (DE3)	Ochrobactrum sp. T.	pET30a-a6	117 kDa	Degradation of TBBPA	[65]
15	Dehydrogenase	E. coli BL21	Pseudomonas umsongensis YCIT1612	pET28a(−)	729 bp/25.6 kDa	Epoxide assimilating opening reaction	[66]
16	Dehydrogenase	E. coli BL21	Bacillus sp. GZT	pET30a(+)	565 bp/63.4 kDa	Degradation of TBP (tribromophenol)	[67]
17	Dehydrogenase	E. coli K-12 NM522	Rhizobium sp.	pUC19	6500 bp	Degradation of halo alkanoate	[68]
18	Hydrolase CPD	E. coli BL21 (DE3)	Paracoccus sp. TRP	pET-32a (+)	51.7 kDa	Degradation of chlorpyrifos	[69]
19	Hydrolase OPH	E. coli DH5α	Pseudomonas diminuta	pQE30	35kDa	Bioremediation of organophosphorus pesticides	[70]
20	Opd A; Opd E	E. coli BL21	Leuconostoc mesenteroides WCP307	pET32a(+)	930 bp/35 kDa; 894 bp/33 kDa	Biodegradation of organophosphorus insecticides	[71]
21	6-OCH-CoA hydrolase	E. coli	Geobacter metallireducens	pCR^®T7/CT-TOPO^®	41.9 kDa	Degradation of aromatic compounds	[72]