Anti-Diabetic Effects of Cucurbitacins from Ibervillea lindheimeri on Induced Mouse Diabetes

Table 2

Results of docking analysis of cucurbitacins 1, 2, and 4 with (A) the active site of AKT and (B) cystathionine β-synthetase (CBS) sites of AMPK.

Site of interaction	Cucurbitacin 1		Cucurbitacin 2		Cucurbitacin 4
	B.E. (kcal/mol)	Kd (µM)	B.E. (kcal/mol)	Kd (µM)	B.E. (kcal/mol)	Kd (mM)
(A) AKT
ATP binding site	−7.97	1.43	−6.05	37.05	−3.05	5.83
(B) AMPK
Cbs1	−6.46	18.4	−9.08	0.22	−3.23	4.26
Cbs3	−6.31	23.57	−7.32	4.11	−3.3	N.B.
Cbs4	−6.36	21.83	−3.46	2,890	−1.73	54.39

Note. B.E. = binding energy and K_d = estimated dissociation constant. N.B. = no binding found.