Development and Validation of an Ion-Pair HPLC-UV Method for the Quantitation of Quinoline and Indoloquinoline Alkaloids in Herbal and Pharmaceutical Antimalarial Formulations

Figure 1

HPLC chromatograms of reference standards and samples using the developed method. (a) Chromatogram showing the optimum separation of the reference standards, namely, cinchonidine, cinchonine, quinidine, quinine, dihydroquinidine, dihydroquinine, and cryptolepine using the developed method. (b) Chromatogram showing the presence of quinine, cinchonidine, and dihydroquinine in an API sample. (c) Chromatogram showing the presence of quinine, quinidine, and dihydroquinidine in a QBPAF. (d) Chromatogram showing the presence of quinine, quinidine, and cryptolepine in a HAMP.

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