Development and Validation of an Ion-Pair HPLC-UV Method for the Quantitation of Quinoline and Indoloquinoline Alkaloids in Herbal and Pharmaceutical Antimalarial Formulations
Table 1
Summary of validation results for the developed method.
Cinchonidine
Cinchonine
Quinidine
Quinine
Dihydroquinidine
Dihydroquinine
Cryptolepine
Retention times
5.10 ± 0.14
6.08 ± 0.13
7.25 ± 0.11
8.12 ± 0.23
9.35 ± 0.30
10.19 ± 0.27
12.32 ± 0.48
LDR (μg/mL)
10–200
10–200
10–200
50–1000
10–200
10–200
10–200
r 2
0.9995
0.9976
0.9996
0.9985
0.9990
0.9994
0.9984
LOD (μg/mL)
3.83
8.18
3.30
32.01
5.12
3.99
6.74
LOQ (μg/mL)
11.61
24.79
10.00
97.00
15.52
12.11
20.42
Intraday precision (% RSD)
1.13
0.89
0.89
0.79
0.90
1.13
0.45
Interday precision (% RSD)
0.76
0.68
0.73
0.79
0.67
0.83
0.55
Accuracy ± SD (% recovery)
99.53 ± 0.7103
99.73 ± 0.8459
100.1 ± 0.9295
100.2 ± 1.157
99.93 ± 1.1016
99.50 ± 0.9031
99.69 ± 0.6641
Robustness
No significant differences in the contents of analytes from the two columns
Stability of analyte solutions (hrs.)
6
6
9
9
6
6
12
Linear dynamic range (LDR), coefficient of correlation (r2), LOD, and LOQ were determined from linear regression curves.
