Journal of Chemistry

Research Article

Antioxidant and Anti-Inflammatory Activities of Phytochemicals from Ruellia tuberosa

Table 2

¹H NMR (500 MHz) and ¹³C NMR (125 MHz) NMR spectral data of compounds 4, 5, 7, and 8 in CDCl₃ and 1-3 and 6 in CD₃OD.


Pos.	Cpd. 1 (CD₃OD)Myricitrin		Cpd. 2 (CD₃OD)Afzelin		Cpd. 3 (CD₃OD)Apigenin		Cpd.6 (CD₃OD)Hispidulin
Pos.	δ_H ppm (J, Hz)	δ_C ppm	δ_H ppm (J, Hz)	δ_C ppm	δ_H ppm (J, Hz)	δ_C ppm	δ_H ppm (J, Hz)	δ_C ppm

2		159.5		159.3		166.3		165.1
3		136.3		136.2	6.61 (1H, s)	103.8	6.65 (1H, s)	103.5
4		179.7		179.6		183.9		183.5
5		163.2		163.2		163.2		153.9
6	6.22 (1H, d, 2.0)	99.8	6.22 (1H, d, 2)	99.9	6.23 (1H, d, 2.0 Hz)	100.1		132.1
7		165.9		166.0		166.0		157.7
8	6.39 (1H, d, 2.0)	94.7	6.39 (1H, d, 2)	94.8	6.48 (1H, d, 2.0 Hz)	95.0	6.63 (1H, s)	94.6
9		158.5		158.6		159.4		153.9
10		105.9		105.9		105.3		105.7
1′		121.9		122.7		123.3		123.1
2′	6.97 (1H, s)	109.6	7.77 (1H, d, 9.0)	131.9	7.87 (1H, d, 7.0)	129.4	7.97 (1H, d, 8.5)	129.2
3′		146.9	6.95 (1H, d, 8.5)	116.5	6.95 (2H, d, 7.0)	117.0	7.07(1H, d, 8.5)	116.8
4′		137.9		161.6		162.7		161.9
5′		146.9	6.95 (1H, d, 8.5)	116.5	6.95 (2H, d, 7.0)	117.0	7.07(1H, d, 8.5)	116.8
6′	6.97 (1H, s)	109.6	7.77 (1H, d, 9.0)	131.9	7.87 (1H, d, 7.0)	129.4	7.97 (1H, d, 8.5)	129.2
OCH₃							3.80 (3H, s)	60.6

Rha unit
1″	5.34 (1H, d, 1.5)	103.6	5.39 (1H, d, 1.5)	103.5
2″	4.23 (1H, s)	71.9	4.24 (1H; dd; 3.5; 1.5)	72.0
3″	3.81 (1H, dd, 9.3; 3.3)	72.1	3.73 (1H; dd; 9.0; 3.5)	72.1
4″	3.33 (1H, m)	73.3	3.34 (1H; m)	73.2
5″	3.54 (1H, m)	72.0	3.34 (1H; m)	71.9
6″	0.99 (3H, d, 6.0)	17.7	0.94 (3H; d; 5.5)	17.6

Pos.	Cpd 4 (CDCl₃)Taraxerol		Cpd 5 (CDCl3)Lupeol
Pos.	δ_H ppm (J, Hz)	δ_C ppm	δ_H ppm (J, Hz)	δ_C ppm
1	1.63 (2H, m)	37.7		38.8
2	1.61 (2H, m)	27.1	1.62 (2H, m)	27.5
3	3.19 (1H, dd, 10.5 và 3.5)	79.0	3.19 (1H,t, 10.5)	79.0
4		38.7		38.9
5	0.78 (1H, dd, 11.5 và 2.5)	55.5	0.67 (1H, t, 9.8)	55.4
6	1.62 (1H, m); 1.47 (1H, m)	18.8	1.38 (2H, m)	18.4
7	2.03 (1H, td, 12.5 and 3.0);1.38 (1H, m)	41.3		34.3
8		39.0		40.9
9	1.40 (1H, m)	49.3	1.25 (1H, m)	50.5
10		37.5		37.2
11	1.63 (1H, m); 1.49 (1H, m)	17.5		21.0
12	1.32 (1H, m); 0.97 (1H, m)	37.7		25.2
13		35.8		38.1
14		158.1		42.9
15	5.53 (1H, dd, 3.5 and 8.5)	116.8		27.5
16	1.91 (1H, dd, 14.5 and 3.0)1.58 (1H, m)	36.7		35.6
17		38.0		43.0
18	0,96 (1H,m)	48.7	1.35 (1H, m)	48.4
19	1.38 (1H, m); 1.02 (1H, m)	35.1	2.38 (1H, m)	48.0
20		28.8		151.0
21	1.62 (1H, m); 1.54 (1H, m)	33.7		29.9
22	1.38 (1H, m); 1.27 (1H, m)	33.1		40.0
23	0.97 (3H, s)	28.0	0.98 (3H, s)	28.0
24	0.80 (3H, s)	15.4	0.76 (3H, s)	15.4
25	0.92 (3H, s)	15.4	0.83 (3H, s)	16.1
26	0.82 (3H, s)	29.8	1.2 (3H, s)	16.0
27	1.09 (3H, s)	25.9	0.99 (3H, s)	14.6
28	0.91 (3H, s)	29.9	0.79 (3H, s)	18.0
29	0.95 (3H, s)	33.3	4.57 (1H, s)4.69 (1H, s)	109.3
30	0.91 (3H, s)	21.3	1.68 (3H, s)	19.3

Pos.	Cpd. 7 (CDCl₃) PhysalinE		Cpd.8 (CDCl3) PhysalinD
Pos.	δ_H ppm (J, Hz) 500 MHz	δ_C ppm 125 MHz	δ_H ppm (J, Hz)	500 MHz δ_C ppm125 MHz

1		207.7		206.6
2	5.82 (1H, dd, 10.4 and 2.4)	128.6	5.87 (1H, dd, 10.5 and 2.0)	127.5
3	6.70 (1H, dd, 10.2 and 2.8)	144.4	6.66 (1H, dd, 10.5 and 2.0)	143.2
4	3.33 (1H, d, 23.6)	36.5	2.06 (1H, m)3.30 (1H, d, 20.0)	35.3
5		778.4		77.2
6		27.6	3.66 (1H, d, 2.5)	73.3
7		74.8	2.04 (1H, m)2.06 (1H, m)	38.3
8	3.27 (1H, dd, 10.4 and 8.8)	39.8	2.44 (1H, dd, 12.0 and 3.5)	35.3
9		27.6	3.07 (1H, dd, 10.5 and 8.5)	30.9
10		55.6		54.5
11	1.13 (1H, m, H-11)	25.9	1.86 (1H, t, 14.0)1.17 (1H, m)	25.6
12		26.4	1.53 (1H, dd, 17.05 and 10.5)2.24 (1H, s)	26.1
13		80.9		80.6
14		108.8		107.5
15		210.4		208.2
16	2.59 (1H, s)	56.5	2.40 (1H, m)	55.7
17		80.9		80.9
18		173.9		172.3
19	1.27 (3H, s)	14.3	1.29 (3H, s)	13.8
20		82.2		80.9
21	1.97 (3H, s)	22.2	2.01 (3H, s)	21.3
22	4.61 (1H, t, 2.0)	78.9	4.55 (1H, t, 2.0)	77.2
23		32.1	2.11 (1H, m)2.07 (1H, m)	32.8
24		31.8		30.4
25	2.80 (1H, d, 4.0)	51.5	2.48 (1H, d, 4.0)	50.8
26		170.2		167.3
27	4.43 (1H, dd, 12.8 and 4.4)3.80 (1H, d, 13.6)	61.8	4.50 (1H, dd, 13.5 and 4.5)3.77 (1H, d, 13.5)	60.5
28	1.32 (3H, s)	26.4	1.27 (3H, s)	26.5