Simultaneous Determination of Content and Antioxidant Activity of Five Components in Hibiscus mutabilis L by HPLC-QAMS

Table 3

Results of sample recovery of each component.

Components	Original quantity/μg	Adding quantity/μg	Measured quantity/μg	Recovery rate/%	Average recovery rate/%	RSD; /%
Protocatechuic acid	15.221	15.09	30.098	98.59	98.85	1.30
	14.954	15.09	29.525	96.56
	13.113	15.09	27.993	98.61
	15.228	15.09	30.227	99.40
	15.876	15.09	30.996	100.20
	14.953	15.09	30.006	99.75
Catechin	153.843	154.8	304.799	97.52	97.60	0.88
	155.116	154.8	304.115	96.25
	149.375	154.8	300.185	97.42
	150.610	154.8	301.364	97.39
	148.603	154.8	300.706	98.26
	148.226	154.8	301.102	98.76
Rutin	1921.910	1965	3903.125	100.83	98.80	1.81
	1866.231	1965	3810.418	98.94
	1773.257	1965	3684.005	97.24
	1833.025	1965	3732.736	96.68
	2006.813	1965	3990.11	100.93
	1992.604	1965	3922.047	98.19
Quercetin	61.705	62.4	122.022	96.66	96.97	1.11
	57.359	62.4	117.072	95.69
	55.005	62.4	115.081	96.28
	61.917	62.4	122.972	97.84
	62.532	62.4	122.899	96.74
	60.277	62.4	121.816	98.62
Luteolin	54.998	50.5	105.221	99.45	99.37	1.38
	49.253	50.5	98.403	97.33
	46.071	50.5	95.733	98.34
	54.774	50.5	105.915	101.27
	55.163	50.5	105.534	99.74
	53.275	50.5	103.806	100.06