Research Article
Simultaneous Determination of Content and Antioxidant Activity of Five Components in Hibiscus mutabilis L by HPLC-QAMS
Table 3
Results of sample recovery of each component.
| Components | Original quantity/μg | Adding quantity/μg | Measured quantity/μg | Recovery rate/% | Average recovery rate/% | RSD /% |
| Protocatechuic acid | 15.221 | 15.09 | 30.098 | 98.59 | 98.85 | 1.30 | 14.954 | 15.09 | 29.525 | 96.56 | 13.113 | 15.09 | 27.993 | 98.61 | 15.228 | 15.09 | 30.227 | 99.40 | 15.876 | 15.09 | 30.996 | 100.20 | 14.953 | 15.09 | 30.006 | 99.75 |
| Catechin | 153.843 | 154.8 | 304.799 | 97.52 | 97.60 | 0.88 | 155.116 | 154.8 | 304.115 | 96.25 | 149.375 | 154.8 | 300.185 | 97.42 | 150.610 | 154.8 | 301.364 | 97.39 | 148.603 | 154.8 | 300.706 | 98.26 | 148.226 | 154.8 | 301.102 | 98.76 |
| Rutin | 1921.910 | 1965 | 3903.125 | 100.83 | 98.80 | 1.81 | 1866.231 | 1965 | 3810.418 | 98.94 | 1773.257 | 1965 | 3684.005 | 97.24 | 1833.025 | 1965 | 3732.736 | 96.68 | 2006.813 | 1965 | 3990.11 | 100.93 | 1992.604 | 1965 | 3922.047 | 98.19 |
| Quercetin | 61.705 | 62.4 | 122.022 | 96.66 | 96.97 | 1.11 | 57.359 | 62.4 | 117.072 | 95.69 | 55.005 | 62.4 | 115.081 | 96.28 | 61.917 | 62.4 | 122.972 | 97.84 | 62.532 | 62.4 | 122.899 | 96.74 | 60.277 | 62.4 | 121.816 | 98.62 |
| Luteolin | 54.998 | 50.5 | 105.221 | 99.45 | 99.37 | 1.38 | 49.253 | 50.5 | 98.403 | 97.33 | 46.071 | 50.5 | 95.733 | 98.34 | 54.774 | 50.5 | 105.915 | 101.27 | 55.163 | 50.5 | 105.534 | 99.74 | 53.275 | 50.5 | 103.806 | 100.06 |
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