Simultaneous Determination of Content and Antioxidant Activity of Five Components in Hibiscus mutabilis L by HPLC-QAMS

Table 5

Effects of different instruments and columns on relative correction factors.

Instrument	Chromatographic column	Relative correction factor
		Protocatechuic acid	Catechin	Rutin	Luteolin
Agilent 1260	Agilent ZORBAX SB-C18	0.9045	0.0917	0.1996	1.3902
	Agilent TC-C18	0.9228	0.0931	0.2011	1.4133
	Phenomenex C18	0.9134	0.0938	0.2002	1.4405
Shimadzu LC-2030	Agilent ZORBAX SB-C18	0.8904	0.0892	0.1970	1.4007
	Agilent TC-C18	0.9173	0.0916	0.2003	1.4328
	Phenomenex C18	0.9008	0.0905	0.1975	1.4216
Average		0.9082	0.0917	0.1993	1.4165
RSD/%		1.31	1.83	0.83	1.35