Simultaneous Determination of Content and Antioxidant Activity of Five Components in Hibiscus mutabilis L by HPLC-QAMS

Table 6

Effects of different column temperatures on relative correction factors.

Column temperature	Relative correction factor
	Protocatechuic acid	Catechin	Rutin	Luteolin
25°C	0.9026	0.0944	0.1999	1.4425
30°C	0.9211	0.0928	0.2023	1.4298
35°C	0.9135	0.0951	0.2021	1.4469
Average	0.9124	0.0941	0.2014	1.4397
RSD/%	1.02	1.25	0.66	0.62