Simultaneous Determination of Content and Antioxidant Activity of Five Components in Hibiscus mutabilis L by HPLC-QAMS

Table 8

Relative retention values of various constituents.

Instrument	Chromatographic column	Relative retention values
		Protocatechuic acid	Catechin	Rutin	Luteolin
Agilent 1260	Agilent ZORBAX SB-C18	2.9839	1.9830	1.1995	0.9527
	Agilent TC-C18	2.9846	1.9964	1.2021	0.9608
	Phenomenex C18	2.9735	1.9803	1.1992	0.9588
Shimadzu LC-2010	Agilent ZORBAX SB-C18	2.9817	1.9932	1.2037	0.9521
	Agilent TC-C18	2.9742	1.9837	1.1926	0.9594
	Phenomenex C18	2.9801	1.9929	1.2134	0.9625
Average		2.9797	1.9883	1.2018	0.9577
RSD/%		0.16	0.34	0.57	0.45