Total Active Compounds and Mineral Contents in Wolffia globosa

Table 2

Validation results of the analysis of ferulic acid, luteolin 7-O-β-D-glucoside, and kaempferol in the W. globosa extract by the HPLC method.

Parameters	Ferulic acid	Luteolin 7-O-β-D-glucoside	Kaempferol
Range (μg/milliliter)	4–15	20–100	4–15
Linearity
Regression equation	y = 73.341x − 65.298	y = 59.273x − 68.148	y = 57.638x − 40.183
Coefficient of determination	0.9988	0.9951	0,9989
Percentage recovery	102.59 ± 1.57	98.77 ± 4.67	101.39 ± 4.48
Precision (%RSD)
Within-day	0.45–1.39	1.02–1.72	1.81–2.54
Between-day	1.81–5.38	2.99–3.64	3.82–6.20
Limit of detection (μg/milliliter)	2.0	2.5	2.0
Limit of quantitation (µg/milliliter)	4.0	10.0	4.0
Retention time (minute)	11.2	11.8	15.3
Wavelength detection (λ, nm)	340	340	340