Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings

Table 1

The diameter of the inhibition zone (mm) of AgNPs against the selected bacterial strains.

Bacterial strains	Control		AgNP concentration (μg·mL^−1)
	Pos.	Neg.	100	75	50	25	12.5
E. coli	18 ± 0.18^a	10 ± 0.0^b	19 ± 0.22^a	18 ± 0.20^a	18 ± 0.19^a	17 ± 0.16^a	10 ± 0.00^b
P. aeruginosa	10 ± 0.0^c	10 ± 0.0^c	28 ± 0.29^a	27 ± 0.26^a	27 ± 0.23^a	24 ± 0.23^a	13 ± 0.11^b
Klebsiella spp	10 ± 0.0^c	10 ± 0.0^c	25 ± 0.44^a	24 ± 0.41^a	21 ± 0.25^b	17 ± 0.25^b	10 ± 0.00^c
Proteus spp	12 ± 0.10^b	10 ± 0.0^c	26 ± 0.34^a	19 ± 0.31^b	17 ± 0.25^b	16 ± 0.14^b	10 ± 0.00^c

Values followed by the same letter in the row do not differ by the Tukey test at 5% probability. Average of three replicates. Tetracycline: positive control. Saline: negative control. a = nonsignificant; b = significant; c = highly significant at the 0.05 level.