Insights into Sulfhemoglobin Detection: UV-Vis and Fluorescence Spectroscopy Correlations

Figure 2

Sulfheme complex spectra as a function of hydrogen sulfide concentration (165 μM–1155 μM) and time. (a) Sulfmyoglobin product spectra. (b) Sulfmyoglobin 618 nm absorbance as a function of initial hydrogen sulfide concentration over a period of four hours. (c) Sulfhemoglobin product spectra and (d) sulfhemoglobin 623 nm absorbance as a function of initial hydrogen sulfide concentration over a period of four hours.

(a)

(b)

(c)

(d)