In Vivo siRNA Delivery and Rebound of Renal LRP2 in Mice

Figure 3

siLRP2 reduced renal mRNA expression in vivo. (a) At 24 h after 7.5 mcg/gm siLRP2 administration, expression of LRP2 was not reduced in mouse kidney ( control, siLRP2). (b) At 3 h after siLRP2 administration, mice receiving 7.5 mcg/gm did not have LRP2 expression reduction. Mice receiving 15 mcg/gm had 25.2% lower LRP2 expression as compared to control . Mice receiving a modified in vivo scrambled siRNA (siSCR) did not have LRP2 expression reduction ( per group). (c) At 6 h after 15 mcg/gm siLRP2 administration, mice receiving siLRP2 had lower expression of LRP2 as compared to control mice. At 12 h after 15 mcg/gm siLRP2, mice had increased expression as compared to control ( control, siLRP2 at 6 and 12 h). The 3 h expression data from Figure 3(b) is pictured for reader ease. All siRNA was administered via tail vein and prepared in PBS vehicle. as compared to the control or PBS vehicle.

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