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Experimental Diabesity Research
Volume 4, Issue 2, Pages 125-132
http://dx.doi.org/10.1155/EDR.2003.125

Hyperglycemia-Induced Protein Kinase C Activation Inhibits Phagocytosis of C3b- and Immunoglobulin G–Opsonized Yeast Particles in Normal Human Neutrophils

Department of Integrative Medical Biology, Section for Histology and Cell Biology, Umeå University, Umeå SE-901 87, Sweden

Received 11 February 2003; Accepted 8 May 2003

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate the effects of elevated glucose concentrations on complement receptor– and Fcγ receptor–mediated phagocytosis in normal human neutrophils. D-Glucose at 15 or 25 mM dose-dependently inhibited both complement receptor– and Fcγ receptor– mediated phagocytosis, as compared to that at a normal physiological glucose concentration. The protein kinase C (PKC) inhibitors GF109203X and Go6976 both dose dependently and completely reversed the inhibitory effect of 25 mM D-glucose on phagocytosis. Complement receptor– mediated phagocytosis was dose-dependently inhibited by the cell permeable diacylglycerol analogue 1,2-dioctanoylsn- glycerol (DAG), an effect that was abolished by PKC inhibitors. Furthermore, suboptimal inhibitory concentrations of DAG and glucose showed an additive inhibitory effect on complement receptor–mediated phagocytosis. The authors conclude that elevated glucose concentrations can inhibit complement receptor and Fcγ receptor–mediated phagocytosis in normal human neutrophils by activating PKCα and/or PKCβ, an effect possibly mediated by DAG.