Acute-Phase Serum Amyloid A as a Marker of Insulin Resistance in Mice
Figure 5
Effects of recombinant A-SAA and
TNFα on gene expression in differentiated 3T3-L1 adipocytes. (a)
Dose-dependent induction of Saa3 and Ccl2 by recombinant A-SAA.
Differentiated 3T3-L1 adipocytes were washed with PBS and starved overnight in
DMEM containing 0.1% BSA. Subsequently, cells were treated for 24 hours with
various concentrations of recombinant A-SAA (0, 0.125 1.25, 12.5, 25 g/mL).
(b) Regulation of signaling genes by A-SAA and TNFα: Differentiated 3T3-L1 adipocytes were treated as described under (a) and subsequently incubated with A-SAA (12.5 g/mL, closed bars) and TNFα (10 ng/mL, hatched bars) for 24 hours. (c) Effect of polymyxin B (PMB) on induction of Saa3 and Ccl2. PMB at 10 g/mL was added alone or in combination with
lipopolysaccharide (LPS, 2.5 ng/mL) and A-SAA (1.25 g/mL), respectively. Experimental
conditions were as described under (a). Copy numbers are relative to one copy of TBP mRNA. Representative
data from at least three independent experiments are shown. Data are mean ±
standard deviation relative to untreated controls.
