Acute-Phase Serum Amyloid A as a Marker of Insulin Resistance in Mice

Table 1

Expression of SAA isoforms and SAA inducers in liver, adipose tissue and skeletal muscle of HFD-fed mice. Data are reported as copy numbers relative to 10⁴ copies of house keeper. Numbers of animals (𝑛) are in parentheses. Data are mean ±SEM; ^*𝑃<.05 versus Chow, ^**𝑃<.01 versus Chow, ^(a)𝑃<.05 versus 1 w HFD, ^(b)𝑃<.01 versus 1 w HFD; two-tailed Student’s t-test.

Experimental Groups	Chow	1 w HFD	16 w HFD
	Copy number	Copy number	Copy number
Liver
Saa1	20,885 ± 3,746 (5)	80,450 ± 27,367 (6)	168,070 ± 24,441 (5)^**(a)
Saa2	96,460 ± 16,927 (5)	370,729 ± 150,376 (6)	998,406 ± 164,697 (5)^**(a)
Saa3	18,824 ± 4,753 (6)	16,098 ± 3,374 (6)	31,641 ± 7,118 (6)
Il1b	1,216 ± 138 (6)	813 ± 87 (6)^*	1,885 ± 255 (6)^*(b)
Il6	30 ± 5 (6)	22 ± 4 (6)	21 ± 3 (6)
Tnf	296 ± 65 (6)	339 ± 51 (6)	1,159 ± 142 (6)^**(b)
Adipose tissue
Saa1	262 ± 86 (6)	70 ± 21 (6)	78 ± 17 (6)
Saa2	1,544 ± 1,035 (5)	615 ± 170 (4)	355 ± 75 (5)
Saa3	5,566 ± 1,086 (5)	60,924 ± 25,363 (4)^*	48,600 ± 14,279 (5)^*
Il1b	261 ± 88 (5)	97 ± 21 (4)	384 ± 142 (5)
Il6	253 ± 105 (6)	139 ± 24 (6)	618 ± 352 (6)
Tnf	648 ± 189 (6)	1,015 ± 119 (6)	6,009 ± 488 (6)^**(b)
Skeletal muscle
Saa1	24 ± 4 (6)	23 ± 6 (6)	36 ± 4 (6)
Saa2	92 ± 18 (6)	75 ± 12 (6)	96 ± 16 (6)
Saa3	44 ± 21 (6)	65 ± 23 (6)	1,196 ± 210 (6)^**(b)
Il1b	34 ± 6 (6)	41 ± 8 (6)	45 ± 5 (6)
Il6	88 ± 10 (6)	96 ± 13 (6)	56 ± 5 (6)^*(a)
Tnf	60 ± 20 (6)	115 ± 30 (6)	122 ± 7 (6)^*