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Experimental Diabetes Research
Volume 2008, Article ID 473603, 7 pages
Research Article

AGEs and Glucose Levels Modulate Type I and III Procollagen mRNA Synthesis in Dermal Fibroblasts Cells Culture

1Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 105 Splaiul Independentei, 050097 Bucharest, Romania
2Molecular Biology Center, Faculty of Biology, University of Bucharest, 91–95 Splaiul Independentei, 050095 Bucharest, Romania

Received 10 July 2007; Revised 3 February 2008; Accepted 14 February 2008

Academic Editor: Andreas Pfützner

Copyright © 2008 Serban Iren Andreea et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In the dermis, fibroblasts play an important role in the turnover of the dermal extracellular matrix. Collagen I and III, the most important dermal proteins of the extracellular matrix, are progressively altered during ageing and diabetes. For mimicking diabetic conditions, the cultured human dermal fibroblasts were incubated with increasing amounts of AGE-modified BSA and D-glucose for 24 hours. The expression of procollagen 2(I) and procollagen 1(III) mRNA was analyzed by quantitative real-time PCR. Our data revealed that the treatment of fibroblasts with AGE-modified BSA upregulated the expression of procollagen 2(I) and procollagen 1(III) mRNA in a dose-dependent manner. High glucose levels mildly induced a profibrogenic pattern, increasing the procollagen 2(I) mRNA expression whereas there was a downregulation tendency of procollagen 1(III) mRNA.