AGEs and Glucose Levels Modulate Type I and III Procollagen mRNA Synthesis in Dermal Fibroblasts Cells Culture

Figure 1

Fluorescence emission spectra in RFU (relative fluorescence units)/1 mg BSA; 6 weeks incubation of BSA (100 mg/mL) at 37^˚C in PBS 10 mM pH 7.4; curve (1) unglycated BSA (control); curve (2) BSA + 1.6 M D-glucose (AGE-modified BSA). (a) Fluorescence emission spectra of samples at 335 nm excitation. (b) Fluorescence emission spectra of samples at 370 nm excitation.

(a)

(b)